Charting the subcellular proteome
نویسندگان
چکیده
منابع مشابه
Subcellular localization of the yeast proteome.
Protein localization data are a valuable information resource helpful in elucidating eukaryotic protein function. Here, we report the first proteome-scale analysis of protein localization within any eukaryote. Using directed topoisomerase I-mediated cloning strategies and genome-wide transposon mutagenesis, we have epitope-tagged 60% of the Saccharomyces cerevisiae proteome. By high-throughput ...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2017
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.4359